Lipoxin A4-d5 (LXA4-d5) contains five deuterium atoms at the 19, 19', 20, 20, and 20 positions. It is intended for use as an internal standard for the quantification of LXA4 by GC- or LC-mass spectrometry (MS). LXA4 is a trihydroxy fatty acid containing a conjugated tetraene, produced by the metabolism of 15-HETE or 15-HpETE with human leukocytes.1 LXA4 is equipotent to leukotriene B4 (LTB4) in inducing superoxide generation in human neutrophils at 0.1 μM.2 LXA4 is associated with several other biological functions including leukocyte activation, chemotaxis effects, natural killer cell inhibition, and monocyte migration and adhesion.2,3,4
1.Serhan, C.N., Nicolaou, K.C., Webber, S.E., et al.Lipoxin A. Stereochemistry and biosynthesisJ. Biol. Chem.261(35)16340-16345(1986)
2.Serhan, C.N., Hamberg, M., and Samuelsson, B.Lipoxins: Novel series of biologically active compounds formed from arachidonic acid in human leukocytesProc. Natl. Acad. Sci. U.S.A.81(17)5335-5339(1984)
3.Ramstedt, U., Serhan, C.N., Nicolaou, K.C., et al.Lipoxin A-induced inhibition of human natural killer cell cytotoxicity: Studies on stereospecificity of inhibition and mode of actionJ. Immunol.138(1)266-270(1987)
4.Maddox, J.F., and Serhan, C.N.Lipoxin A4 and B4 are potent stimuli for human monocyte migration and adhesion: Selective inactivation by dehydrogenation and reductionJ. Exp. Med.183(1)137-146(1996)
