FLAG肽
FLAG肽 性质
| 密度 | 1.470±0.06 g/cm3 (20 ºC 760 Torr) |
|---|---|
| 储存条件 | Keep in dark place,Sealed in dry,Store in freezer, under -20°C |
| 溶解度 | DMSO 中≥50.65 mg/mL；水中≥210.6 mg/mL；乙醇中≥34.03 mg/mL |
| 形态 | 冻干粉 |
| 颜色 | 白色至米白色 |
| 序列 | H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH |
| InChIKey | XZWYTXMRWQJBGX-OVIGALBNNA-N |
FLAG肽 用途与合成方法
Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca 2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%
FLAG肽 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-08 | HY-P0223 | FLAG肽 | 1 mg | 500 | |
| 2024-11-08 | HY-P0223 | FLAG肽 | 5 mg | 900 |
