4-chloro-3-methoxy-7-nitroisochromen-1-one

Caspase-3-IN-2 (Compound 4d) is the inhibitor for α-Chymotrypsin. Caspase-3-IN-2 also exhibits inhibitory activity against HIV protease and caspase 3 with an inhibition rate of 57% and 51% at 100 μM[1].

[1] Bihel F, et al., Synthesis of new 3-alkoxy-7-amino-4-chloro-isocoumarin derivatives as new beta-amyloid peptide production inhibitors and their activities on various classes of protease. Bioorg Med Chem. 2003 Jul 17;11(14):3141-52. DOI:10.1016/s0968-0896(03)00235-9