TBK1 Monoclonal Antibody (Clone 4E6)

TANK-binding kinase 1 (TBK1) is a non-canonical inhibitor of NF-κB kinase (IKK) that has an essential role in regulating inflammatory responses to pathogens. Following activation of toll-like receptors by viral DNA, TBK1 interacts with various partners such as STING, MAVS, and TANK to phosphorylate and activate interferon regulatory factors (IRFs) 3 and 7 as well as DEAD-box helicase 3 X-linked (DDX3X), which leads to transcriptional activation of pro-inflammatory and antiviral genes including interferon (IFN) subtypes α and β. TBK1 induces nuclear translocation of NF-κB to initiate a pro-inflammatory response via phosphorylation of NF-κB inhibitor α (NFκBIα), IKKβ, or NF-κB p65 subunit (RelA). Cytosolic localization of E. coli, Salmonella, and S. pyogenes increases in TBK1^-/- murine embryonic fibroblasts, macrophages, and epithelial cells, suggesting TBK1 maintains vacuolar integrity, which is critical to bacterial clearance. TBK1 phosphorylates the autophagy receptor optineurin to enhance binding of ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers and induce autophagic clearance of S. enterica. It also binds to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 13 (Nsp13), a helicase-triphosphatase and component of the viral replicase-transcriptase complex. Cayman’s TBK1 Monoclonal Antibody (Clone 4E6) can be used for ELISA, Immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes TBK1 at approximately 84 kDa.

[1] J L POMERANTZ  D B. NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase.[J]. EMBO Journal, 1999, 18 23: 6694-6704. DOI: 10.1093/emboj/18.23.6694
[2] YASUO TANAKA  Zhijian J C. STING Specifies IRF3 Phosphorylation by TBK1 in the Cytosolic DNA Signaling Pathway[J]. Science Signaling, 2012, 5 214. DOI: 10.1126/scisignal.2002521
[3] YUICHIRO TOJIMA. NAK is an IκB kinase-activating kinase[J]. Nature, 2000, 404 6779: 778-782. DOI: 10.1038/35008109
[4] ANDREA L RADTKE. TBK1 protects vacuolar integrity during intracellular bacterial infection.[J]. PLoS Pathogens, 2007, 3 3: e29. DOI: 10.1371/journal.ppat.0030029
[5] PHILIPP WILD. Phosphorylation of the Autophagy Receptor Optineurin Restricts Salmonella Growth[J]. Science, 2011, 333 6039. DOI: 10.1126/science.1205405