Citrulline-specific Probe-biotin

Citrulline-specific probe-biotin is an affinity probe that allows for detection or immobilization of citrullinated proteins through interaction with the biotin ligand. It contains a phenylglyoxal group that selectively labels citrulline over arginine at acidic pH. Citrulline-specific probe-biotin is a biotinylated form of citrulline-specific probe-rhodamine that contains biotin in place of the rhodamine fluorophore.

Citrulline-specific probe-biotin is a biotinylated probe for detection of citrulline. Citrulline is the hydrolysis product of arginine catalyzed by protein arginine deiminase (PAD). PAD is abnormally activated in many diseases, leading to increased citrulline levels. Citrulline-specific probe-biotin is a biological probe that can identify diseases showing abnormal increases in PAD activity and may be effectively used in animal models of ulcerative colitis[1].

[1] Bicker K L, et al. Seeing citrulline: development of a phenylglyoxal-based probe to visualize protein citrullination[J]. Journal of the American Chemical Society, 2012, 134(41): 17015-17018. DOI:10.1021/ja308871v
[2] MICHAEL R PITTER. PAD4 controls tumor immunity via restraining the MHC class II machinery in macrophages.[J]. Cell reports, 2024, 43 3: 113942. DOI: 10.1016/j.celrep.2024.113942