MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

Product NameMCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
CAS140430-53-1
MFC49H68N14O15
MW1093.15
EINECS
MOL File140430-53-1.mol

Chemical Properties

Density	1.48±0.1 g/cm3(Predicted)
storage temp.	−20°C
solubility	20% acetic acid: 1mg/mL
form	Solid
pka	12.99±0.46(Predicted)
color	Light yellow to yellow
Appearance	Yellow Powder
InChIKey	RQASLOJQHHELIQ-QJSKOQLASA-N

Safety Information

WGK Germany	3
Storage Class	11 - Combustible Solids

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Description

7-Methoxycoumarin-4-acetyl-P-L-G-L-β-(2,4-dinitrophenylamino)A-R amide is a fluorogenic substrate for matrix metalloproteinases (MMPs). It is ceaved by an MMP to release the fluorescent moiety 7-methoxycoumarin-4-acetyl (Mca), which can be used to quantify MMP activity.

Uses

MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues[1][2].

Biological Activity

Cell permeable: no', 'Primary Target
Fluorogenic substrate for matrix metalloproteinases (MMPs)', 'Product does not compete with ATP.', 'Reversible: no', 'kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M-¹ sec-¹ for MMP-2

References

[1] Oneda H, et al. Substrate-dependent activation of thermolysin by salt. Biosci Biotechnol Biochem. 2004 Aug;68(8):1811-3. DOI:10.1271/bbb.68.1811
[2] Oneda H, et al. Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin). J Biochem. 2003 May;133(5):571-6. DOI:10.1093/jb/mvg073

Sequene

Mca-PLGL-Dap(Dnp)-AR-NH2