The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.
1,2,3 PtdIns-
(3,4,5)-
P
3-
biotin is an affinity probe which allows the PIP
3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-
(3,4,5)-
P
3-
biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-
(3,4,5)-
triphosphate phospholipids, such as phosphatidylinositol 3-
kinase, PTEN, or PH-
domain-
containing proteins.
1. Majerus, P.W. Inositol phosphate biochemistry Annu. Rev. Biochem. 61,225-250(1992).
2. Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins Annu. Rev. Pharmacol. Toxicol. 36,481-509(1996).
3. Rückle, T., Schwarz, M.K., and Rommel, C. PI3Kγ inhibition: Towards an 'aspirin of the 21st century'? Nat. Rev. Drug Discov. 5(11),903-918(2006).
