Description
The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.
1,2,3 PtdIns-
(3,4,5)-
P
3-
biotin is an affinity probe which allows the PIP
3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-
(3,4,5)-
P
3-
biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-
(3,4,5)-
triphosphate phospholipids, such as phosphatidylinositol 3-
kinase, PTEN, or PH-
domain-
containing proteins.
Uses
PtdIns-(3,4,5)-P3-biotin sodium is a Biotin-labeled PtdIns-(3,4,5)-P3 (PI(3,4,5)P3). PI(3,4,5)P3 is a substrate of nuclear phosphatidylinositol 5-phosphatase (PIP5Pase). PI(3,4,5)P3 binds to the N-terminus of RAP1 (repressor activator protein 1) and controls its DNA binding activity[1].
References
1. Majerus, P.W.
Inositol phosphate biochemistry Annu. Rev. Biochem. 61,225-250(1992).
2. Exton, J.H.
Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins Annu. Rev. Pharmacol. Toxicol. 36,481-509(1996).
3. Rückle, T., Schwarz, M.K., and Rommel, C.
PI3Kγ inhibition: Towards an 'aspirin of the 21st century' Nat. Rev. Drug Discov. 5(11),903-918(2006).
