Prostaglandin F2α Ethanolamide-d4

Prostaglandin F2α ethanolamide (PGF2α-EA-d4) contains four deuterium atoms at the 3, 3', 4, and 4' positions. It is intended for use as an internal standard for the quantification of PGF2α-EA by GC- or LC-mass spectrometry. PGF2α-EA is produced by cyclooxygenase 2 (COX-2) metabolism of the endogenous cannabinoid arachidonoyl ethanolamide (AEA) found in brain, liver, and other mammalian tissues.1 AEA can be metabolized by fatty acid amide hydrolase (FAAH) to give free arachidonic acid, which is the well known and conventional substrate for COX enzymes. However, it has also been reported that AEA can be used directly by COX-2 to produce ethanolamide congeners of the classical prostaglandins, including PGE2.2 PGF2α-EA has also been reported to be biosynthesized by this mechanism when AEA was infused into the lung and liver of living mice. PGF2α-EA is a potent dilator (EC50 = 58 nM) of the cat iris sphincter, which is a model system for testing potential intraocular hypotensive agents.3WARNING This product is not for human or veterinary use.

[1] N. BACHUR. FATTY ACID AMIDES OF ETHANOLAMINE IN MAMMALIAN TISSUES.[J]. The Journal of Biological Chemistry, 1965, 97 1: 1019-1024. DOI: 10.1016/s0021-9258(18)97531-9
[2] M YU  C S R  D Ives. Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase-2.[J]. The Journal of Biological Chemistry, 1997, 272 34: 21181-21186. DOI: 10.1074/jbc.272.34.21181
[3] De Souza, F.I., Zumiotti, A.V., and Da Silva, C.F. Neuregulins 1-α and 1-β on the regeneration the peripheral nerves[J]. Acta Ortop Bras.