2'-Deoxy-3'-O-(N'-methylanthraniloyl)adenosine-5'-O-diphosphate (sodium salt)

2'-Deoxy-3'-O-(N'-methylanthraniloyl)adenosine-5'-O-diphosphate (MANT-dADP) is a fluorescent nucleotide derivative.1 It displays an emission maximum of 453 nm upon excitation at 350 nm.1 MANT-dADP (100 µM) decreases inositol phosphate formation in CHO-K1 cells expressing the human purinergic P2Y12 receptor.2 It has been used to study the interaction between cardiac troponin I and myofibrils.3WARNING This product is not for human or veterinary use.

[1] WLODZIMIERZ BUJALOWSKI  Malgorzata M K. Structural Characteristics of the Nucleotide-Binding Site of Escherichia coli Primary Replicative Helicase DnaB Protein. Studies with Ribose and Base-Modified Fluorescent Nucleotide Analogs[J]. Biochemistry Biochemistry, 1994, 33 15: 4682-4694. DOI: 10.1021/bi00181a028
[2] PHILIPP SCHMIDT. Identification of determinants required for agonistic and inverse agonistic ligand properties at the ADP receptor P2Y12.[J]. Molecular Pharmacology, 2013, 83 1: 256-266. DOI: 10.1124/mol.112.082198
[3] LAURA K. GUNTHER. Effect of N-Terminal Extension of Cardiac Troponin I on the Ca2+ Regulation of ATP Binding and ADP Dissociation of Myosin II in Native Cardiac Myofibrils[J]. Biochemistry Biochemistry, 2016, 55 12: 1887-1897. DOI: 10.1021/acs.biochem.5b01059