2'-O-(N'-Methylanthraniloyl)guanosine-5'-O-diphosphate (2’-MANT-GDP) and 3’-MANT-GDP are fluorescent GDP derivatives, which undergo spontaneous isomerization.1 2’(3’)-MANT-GDP selectively inhibits recombinant mammalian adenylyl cyclase 1 (AC1), AC2, AC5, AC6, and bovine lung soluble guanylyl cyclase (sGC; Kis = 430, 2,600, 510, 320, and 4,200 nM, respectively) over B. anthracis edema factor AC toxin, rat testis soluble AC (sAC), and B. pertussis AC toxin (Kis = 37, 48, and 60 µM, respectively). 2’(3’)-MANT-GDP has been used to quantify guanine nucleotide exchange of GTPases.2 A related derivative, 2'(3')-MANT-GTP (Item No. 38469), displays an emission maximum of 442 nm upon excitation at 350 nm.3WARNING This product is not for human or veterinary use.
[1] ANDREAS GILLE. Differential inhibition of adenylyl cyclase isoforms and soluble guanylyl cyclase by purine and pyrimidine nucleotides.[J]. The Journal of Biological Chemistry, 2004, 279 19: 19955-19969. DOI:
10.1074/jbc.m312560200[2] YUPING TAN Qingxiang S. A Mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases.[J]. Bio-protocol, 2021, 11 2: e3886. DOI:
10.21769/bioprotoc.3886[3] HIRATSUKA T. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as subtrates for various enzymes[J]. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983, 742 3: Pages 496-508. DOI:
10.1016/0167-4838(83)90267-4
