RNase Activity Assay Kit (Fluorometric) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time. The assay uses a highly sensitive, specific probe that releases a fluorescent product in the presence of active RNAse. The limit of detection is 0.4 pg RNase/well and limit of quantification is 1.2 pg RNase/well.
Evaluation of RNase contamination is necessary for reagents to be used in experiments with RNA. The RNase Contamination Assay Kit detects general RNase activities including non-enzyme based RNA degradation due to heavy metal contamination in samples and high pH. The assay probe is a fluorescein labeled RNA transcript (300-mer). After incubation with a reagent sample the integrity of the RNA probe is analyzed on denaturing PAGE followed by SYBR Gold staining or preferably by scanning with a FAM/Fluorescein capable imaging system like the Typhoon scanner. Most samples (e.g. enzymes, column fractions from enzyme purifications, unlabeled RNA/DNA samples, buffers, or diluents) can be tested directly. The assay is semi-quantitative and is more sensitive if detergent is present.
1 GGGAAGAGCA UUGUCGAGGG UGAAGUACGG UUCUUGCAGG UUGAACAUCA GCGCGCUCUU
61 ACCUUUCGCU UUCAGUUCUU UAUCCAGCGC CGGGAUCUCU UCCCAGGUUU UUGGCGGGUU
121 CGGCAGCAGA UCUUUGUUAU AAAUCAGCGA UAACGCUUCA ACAGCGAUCG GGUAAGCAAU
181 CAGCUUGCCG UUGUAACGUA CGGCAUCCCA GGUAAACGGA UACAGCUUGU CCUGGAACGC
241 UUUGUCCGGG GUGAUUUCAG CCAACAGGCC AGAUUGAGCG UACUCGACGA AGACUCCCUA
RNase assay kit (Fluoresscence) is a Patent-pending technology detects RNase activity in a convenient and sensitive assay that delivers results in real time. Suitable for testing small sample numbers and can be used to ensure that solutions, tubes, tips, etc. are RNase-free; the kit contains sufficient reagents for 25 reactions.
Quantitative analysis of RNAse activity of purified enzymes
Measurement of RNAse contamination in buffers and samples
Highly sensitive
Specific
The limit of quantification is 1.2 pg RNase/well
The limit of detection is 0.4 pg RNase/well
High Throughput compatible
