2-CHLORO-N-(4-CHLORO-3-DIMETHYLSULFAMOYL-PHENYL)-ACETAMIDE

GSTO1-IN-1 (C1-27) potently inhibits GSTO1 enzyme activity with an IC ₅₀ value of 31 nM. GSTO1-IN-1 also potently competes with 5-chloromethylfluorescein diacetate (CMFDA) for binding to recombinant protein, as well as endogenous GSTO1 in the milieu of a soluble proteome. HCT116 cells treated with GSTO1-IN-1 also show a decrease in cell viability in a dose-dependent manner. GSTO1-IN-1 inhibits the clonogenic survival of HCT116 cells at sub-micromolar concentrations.

IC50: 31 nM (GSTO1)