Methyl-d3-amine hydrochloride

(i) reacting nitromethane with deuterium water under the presence of a base and a phase transfer catalyst to give deuterated nitromethane; and reducing the deuterated nitromethane in an inert solvent to form deuterated methylamine; then optionally reacting the deuterated methylamine with an acid to form METHYL-D3-AMINE HYDROCHLORIDE; or (ii) reacting N-(1,1,1-trideuterated methyl)benzosuccinimide with an acid to form METHYL-D3-AMINE HYDROCHLORIDE.

white crystalline powder

MethylaMine-d3 Hydrochloride is useful in preparation of deuterated diphenylurea derivatives as phosphokinase inhibitors.

Methyl-d3-amine hydrochloride can be used as a heavy isotope labeling reagent, paired with the light isotope reagent methylamine hydrochloride, to achieve relative quantitative analysis of intact N-glycopeptides.

[1]Sun, Z., Ji, G., Wang, G., Wei, L., Zhang, Y., & Lu, H. (2021). One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry. Chemical Communications, 57(34), 4154-4157.