Naphthofluorescein

Product NameNaphthofluorescein
CAS61419-02-1
MFC28H16O5
MW432.42
EINECS634-716-5
MOL File61419-02-1.mol

Chemical Properties

Melting point	189 °C (dec.)(lit.)
Boiling point	755.5±60.0 °C(Predicted)
Density	1.58±0.1 g/cm3(Predicted)
storage temp.	Store at -20°C
solubility	DMF: soluble
form	A crystalline solid
pka	8.82±0.20(Predicted)
color	Purple to purplish red
ex/em	λex 598 nm; λem 671 nm in NaOH 0.1 M; pH 12
BRN	61335
InChI	1S/C28H16O5/c29-17-7-9-19-15(13-17)5-11-23-25(19)32-26-20-10-8-18(30)14-16(20)6-12-24(26)28(23)22-4-2-1-3-21(22)27(31)33-28/h1-14,29-30H
InChIKey	IXQIUDNVFVTQLJ-UHFFFAOYSA-N
SMILES	Oc1ccc2c3Oc4c(ccc5cc(O)ccc45)C6(OC(=O)c7ccccc67)c3ccc2c1
EPA Substance Registry System	Spiro[7H-dibenzo[c,h]xanthene-7,1'(3'H)-isobenzofuran]-3'-one, 3,11-dihydroxy- (61419-02-1)

Safety Information

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
WGK Germany	3
F	8-10
TSCA	TSCA listed
HS Code	29329990
Storage Class	11 - Combustible Solids
Hazard Classifications	Eye Irrit. 2 Skin Irrit. 2 STOT SE 3

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Uses

Naphthofluorescein is a furin inhibitor.

Uses

Naphtofluorescein is a pH-sensitive fluorescent probe, also used for preparation of labels and enzyme substrates.

Definition

ChEBI: Naphthofluorescein is a member of xanthenes.

in vivo

Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks)does not show weight loss or apparent histological abnormalities in the lung, liver, and kidney, or cause cause severe adverse effects for at least 2 weeks in mice^[1].
Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks) strikingly suppresses the tumour growth of subcutaneously injected E0771 cells and significantly attenuates tumour growth of MDA-MB-231 and AsPC-1 cells in immunodeficient mice. In turn, naphthofluorescein does not attenuate tumour growth of FIH-1-depleted MDA-MB-231 cells^[1].
Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks) suppresses tumour growth in human cancer cells in an FIH-1-dependent manner^[1].

Animal Model:	Male C57BL/6J mice (8 weeks)^[1]
Dosage:	100 mg/kg
Administration:	i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks
Result:	Neither showed weight loss or apparent histological abnormalities in the lung, liver, and kidney, or caused cause severe adverse effects for at least 2 weeks in mice.

Animal Model:	Female C57BL/6J mice (E0771) and BALB/c nude mice (HT1080, MDA-MB-231, and AsPC-1) (6 weeks)^[1]
Dosage:	100 mg/kg
Administration:	i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks
Result:	Strikingly suppressed the tumour growth of subcutaneously injected E0771 cells and significantly attenuated tumour growth of MDA-MB-231 and AsPC-1 cells in immunodeficient mice. In turn, naphthofluorescein did not attenuate tumour growth of FIH-1-depleted MDA-MB-231 cells.

Naphthofluorescein Supplier

Naphthofluorescein manufacturers

Related Product Information

Send Inquriy