N-(4-fluorobenzyl)-5-isobutyl-2,2'-bithiazole-4-carboxamide

Isothiafludine, also NZ-4 and W-28-F, is a HBV virus inhibitor that dramatically reduces the HBV DNA level in cells. During the hepatitis B virus (HBV) life cycle, nucleocapsid assembly is essential for HBV replication. Both RNA reverse transcription and DNA replication occur within the HBV nucleocapsid. HBV nucleocapsid is consisted of core protein (HBcAg), whose carboxy-terminal domain (CTD) contains an Arg-rich domain (ARD). The ARD of HBcAg does contribute to the encapsidation of pregenomic RNA (pgRNA).