1-(9-ethyl-9H-carbazol-3-yl)-N-(pyridin-2-ylmethyl)methanamine
1-(9-ethyl-9H-carbazol-3-yl)-N-(pyridin-2-ylmethyl)methanamine 性质
| 沸点 | 505.1±48.0 °C(Predicted) |
|---|---|
| 密度 | 1.14±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 二甲基亚砜：125 mg/mL（396.31 mM） |
| 形态 | 油状物 |
| 酸度系数(pKa) | 7.28±0.20(Predicted) |
| 颜色 | 浅黄至黄色 |
1-(9-ethyl-9H-carbazol-3-yl)-N-(pyridin-2-ylmethyl)methanamine 用途与合成方法
IC50: 3.7 μM (mTh1 cells), 9.2 μM (mTh2 cells)IC50: 26.9 μM (hTh1 cells), 31.6 μM (hTh2 cells)
CMP-5 (0-100 μM; 24-72 hours) is selectively toxic to lymphoma cells, but shows a limited toxicity to normal resting B lymphocytes even after prolonged incubation.CMP-5 (40 μM; 24 hours) decreases p-BTK and pY(416)SRC expression in 60A cells when it compares to the DMSO-treated group.CMP-5 (0-40 μM; 24 hours) preferentially suppresses the proliferation of human Th1 cells over Th2 cells (43 versus 9% inhibition, respectively). The sensitivity of Th1 cells over Th2 cells to PRMT5 inhibition is different, the IC 50 values are 26.9 μM and 31.6 μM in human Th1 cells and Th2 cells, respectively.CMP-5 (25 μM; 24 hours) alone inhibits mouse Th1 cell proliferation by 91%, when added different doses IL-2, IL-2 enhances proliferation and reaches a peak at 5 ng/ml.
Western Blot Analysis
| Cell Line: | 60A cells |
| Concentration: | 40 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited p-BTK and pY(416)SRC protein level. |
Cell Viability Assay
| Cell Line: | Human Th1 cells and Th2 cells |
| Concentration: | 25 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited mouse Th1 cell proliferation, but addition of IL-2 dose-dependently increased cell proliferation. |
