PHOSPHORAMIDEMUSTARD
PHOSPHORAMIDEMUSTARD 性质
| 熔点 | 100-103°C |
|---|---|
| 储存条件 | Hygroscopic, -20°C Freezer, Under inert atmosphere |
| 溶解度 | 可溶于氯仿（少许）、甲醇（少许） |
| 形态 | 固体 |
| 颜色 | 白色至类白色 |
| 稳定性 | 湿气敏感 |
| InChI | 1S/C6H13N.C4H11Cl2N2O2P/c7-6-4-2-1-3-5-6;5-1-3-8(4-2-6)11(7,9)10/h6H,1-5,7H2;1-4H2,(H3,7,9,10) |
| InChIKey | BGTIPRUDEMNRIP-UHFFFAOYSA-N |
| SMILES | ClCCN(P(N)(O)=O)CCCl.NC1CCCCC1 |
PHOSPHORAMIDEMUSTARD 用途与合成方法
Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.
Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs).
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.
Cell Viability Assay
| Cell Line: | SIGCs |
| Concentration: | 0.5 μM, 1 μM, 3 μM, 6 μM |
| Incubation Time: | 48 hours |
| Result: | Reduced cell viability at concentrations of 3 μM and higher. |
RT-PCR
| Cell Line: | SIGCs |
| Concentration: | 3 μM, 6 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Increased DDR gene mRNA expression levels. |
Western Blot Analysis
| Cell Line: | SIGCs |
| Concentration: | 3 μM, 6 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Generally increased DDR proteins. |
Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.
Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).
| Animal Model: | Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor |
| Dosage: | 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg |
| Administration: | Intraperitoneal injection, once daily, for 5 consecutive days |
| Result: | Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg. |
| Animal Model: | Rats |
| Dosage: | 86.0 mg/kg (Pharmacokinetic Analysis) |
| Administration: | Intravenous injection |
| Result: | T 1/2 (15.1 min). |
PHOSPHORAMIDEMUSTARD 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026-09-15 | HY-137316A | 1566-15-0 | 1 mg | 1545 | |
| 2026-09-15 | HY-137316A | PHOSPHORAMIDEMUSTARD | 1566-15-0 | 5mg | 3400 |
