Soluble epoxide hydrolase (sEH) catalyzes the conversion of epoxyeicosatrienoic acids (EpETrEs) to the corresponding DiHETrEs thereby diminishing their vasodilator activity.
1 Inhibitors of sEH may therefore have clinical utility for treating hypertension and systemic inflammation.
2,3 Epoxy fluor 7 is a sensitive fluorescent substrate for soluble epoxide hydrolase (sEH) that can be used to monitor the activity of both human and mouse enzymes.
4 Hydrolysis of the substrate epoxide yields a highly fluorescent product that can be monitored at excitation and emission wavelengths of 330 and 465 nm, respectively. Epoxy fluor 7 is more stable in aqueous solution and offers about 2-
fold better sensitivity than previously used colorimetric substrates such as NEPC.
4
1. Yu, Z., Xu, F., Huse, L.M., et al. Soluble epoxide hydrolase regulates hydrolysis of vasoactive epoxyeicosatrienoic acids Circ. Res. 87(11),992-998(2000).
2. Imig, J.D., Zhao, X., Zaharis, C.Z., et al. An orally active epoxide hydrolase inhibitor lowers blood pressure and provides renal protection in salt-sensitive hypertension Hypertension 46(2),975-981(2005).
3. Schmelzer, K.R., Kubala, L., Newman, J.W., et al. Soluble epoxide hydrolase is a therapeutic target for acute inflammation Proc. Natl. Acad. Sci. USA 102(28),9772-9777(2005).
4. Jones, P.D., Wolf, N.M., Morisseau, C., et al. Fluorescent substrates for soluble epoxide hydrolase and application to inhibition studies Anal. Biochem. 343,66-75(2005).
