Guanosine-5'-O-triphosphoro-γ-1-(5-sulfonic acid)naphthylamidate (GTP-γ-AmNS) is a fluorescent derivative of GTP (Item No. 16060).1,2 It has been used as a substrate for snake venom phosphodiesterases (PDEs) and to monitor tubulin assembly in cell-free assays. GTP-γ-AmNS diplays an emission maximum of 444 nm upon excitation at 320 nm, which is quenched by 1.2-1.4-fold upon cleavage of the α-β phosphoryl bond.1WARNING This product is not for human or veterinary use.
References
[1] STUART E. POLLACK David S A. Fluorescent nucleotide triphosphate substrates for snake venom phosphodiesterase[J]. Analytical biochemistry, 1982, 127 1: Pages 81-88. DOI: 10.1016/0003-2697(82)90147-6 [2] L R YARBROUGH M K. Binding of fluorescent analogs of GTP to the exchangeable nucleotide binding site of tubulin.[J]. The Journal of Biological Chemistry, 1981, 256 1: 112-117.
