2-Amino-5-chloro-4-nitrobenzoic Acid (cas# 34649-02-0) is a useful reagent in the preparation of N-?guanidino substituted 2,?4-?diamino-?5-?carbonylguanidine molecules related to amiloride that inhibits the sodium-?calcium exchanger in rat insulinoma cells (RINm5F) and human platelets.