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Fluorescein isothiocyanate

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Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) <ul> <li>Excitation source: 488 nm spectral line, argon-ion laser</li> <li>Excitation wavelength: 494 nm</li> <li>Emission wavelength: 518 nm</li> <li>Extinction coefficient: &gt; 70,000 M-1cm-1</li> <li>Solubility: Soluble in aqueous buffers at pH &gt; 6</li> <li>Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0</li> </ul>
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