RO 15-4513 is a high affinity benzodiazepine ligand which acts as a partial inverse agonist at recombinant diazepam-sensitive (DS) benzodiazepine α1-, α2-, α3- and α5-GABAA receptors. It has also been shown to activate GABAA R α4 and GABAA R α6. In addition, RO 15-4513 acts as a partial agonist at diazepam-insensitive (DI) benzodiazepine receptors. Furthermore, studies show that ethanol can inhibit the binding of RO 15-4513 to α4/6β3δ GABAA receptors. RO 15-4513 has a similar structure to flumazenil, which differs by an azido group at the C7 position of the imidazo-benzodiazepine ring.
