MitoLite™ reagents are fluorogenic probes that can be used to label mitochondria within live or fixed cells. They are hydrophobic and easily cross live cell membranes to accumulate in mitochondria likely via the mitochondrial membrane potential gradient. MitoLite™ reagents contain a cell-retaining group, which allows them to remain in the mitochondria long-term, and their fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. MitoLite™ NIR FX690 has been used to determine subcellular localization of photosensitizers modified by polyethylene glycol in HepG2 cells. MitoLite™ NIR FX690 displays excitation/emission maxima of 660/692 nm, respectively, and can be used for live and fixed cell applications.
