3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine-threonine kinase that plays an important role in growth factor signaling by phosphorylating and activating a group of kinases, including protein kinase (PK)B, PKA, and certain isoforms of PKC. GSK2334470 is an inhibitor of PDK1 (IC50 = ~10 nM) that even at 100-fold higher concentrations does not affect the activity of 93 other protein kinases, including that of 13 closely related AGC kinase family members. In HEK293 cells, 30 nM - 1 μM GSK2334470 suppresses T-loop phosphorylation and activation of SGK, S6K1, and RSK, as well as the activation of Akt.
