Cayman’s NF-κB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p50) is detected by addition of specific primary antibody directed against NF-κB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The Cayman Chemical NF-κB (human p50) Transcription Factor Assay detects human NF-κB (p50). It will not cross-react with NF-κB (p65).Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
