7-Azido-4-methylcoumarin (AzMC) is a fluorogenic probe useful for the detection of hydrogen sulfide (H2S). The aromatic azide moiety is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with λex = 365nm and λem = 450nm. The probe can be used both to monitor enzymatic production of H2Sin vitroand also to visualize H2S in cells. The probe is highly sensitive and selective for H2S, providing a linear detection rangein vitrobetween 200nm – 100μM H2S with 10μM probe, and reacting selectively with H2S in the presence of mM concentrations of cysteine, homocysteine, glutathionine and many other common biological analytes.Caution: Use of this product with DTT, TCEP and/or biological thiols >25mM should be avoided for maximum efficiency.AzMC is readily soluble in DMSO.
