Uses
7-Azido-4-methylcoumarin (AzMC) is a fluorogenic probe useful for the detection of hydrogen sulfide (H
2S). The aromatic azide moiety is selectively reduced in the presence of H
2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with λex = 365nm and λem = 450nm. The probe can be used both to monitor enzymatic production of H
2S
in vitro and also to visualize H
2S in cells. The probe is highly sensitive and selective for H
2S, providing a linear detection range
in vitro between 200nm – 100μM H
2S with 10μM probe, and reacting selectively with H
2S in the presence of mM concentrations of cysteine, homocysteine, glutathionine and many other common biological analytes.
Caution: Use of this product with DTT, TCEP and/or biological thiols >25mM should be avoided for maximum efficiency.
AzMC is readily soluble in DMSO.
