Base treatment of human seminal plasma extracts converts the 19-
hydroxy PGE compounds present in the sample into the corresponding PGB compounds generating strong conjugated dienone chromophores. This method has been employed to provide a rapid, UV-
based analytical method for PGE quantitation. Due to its chemical stability and strong UV absorbance at 278 nm, 19(R)-
hydroxy PGB
2 is also used as an internal standard in extractions and HPLC to determine recovery of arachidonic acid metabolites.
1,2,3
