Base treatment of human seminal plasma extracts converts the 19-
hydroxy PGE compounds present in the sample into the corresponding PGB compounds generating strong conjugated dienone chromophores. This method has been employed to provide a rapid, UV-
based analytical method for PGE quantitation. Due to its chemical stability and strong UV absorbance at 278 nm, 19(R)-
hydroxy PGB
2 is also used as an internal standard in extractions and HPLC to determine recovery of arachidonic acid metabolites.
1,2,3
1. Antoine, C., Murphy, R.C., Henson, P.M., et al. Time-dependent utilization of platelet arachidonic acid by the neutrophil in formation of 5-lipoxygenase products in platelet-neutrophil co-incubations Biochim. Biophys. Acta 1128,139-146(1992).
2. Borgeat, P., and Picard, S. 19-Hydroxyprostaglandin B2 as an internal standard for on-line extraction-high-performance liquid chromatography analysis of lipoxygenase products Anal. Biochem. 171,283-289(1988).
3. Surette, M.E., Odeimat, A., Palmantier, R., et al. Reverse-phase high-performance liquid chromatography analysis of arachidonic acid metabolites in plasma after stimulation of whole blood ex vivo Anal. Biochem. 216(2),392-400(1994).
