C6 Biotin Ceramide (d18:1/6:0)

C6 Ceramide-biotin is an affinity probe that allows C2 ceramide (d18:1/2:0) to be detected or immobilized through interaction with the biotin ligand. C6 Ceramide-biotin has been used to detect ceramide binding partners, such as inhibitor 2 of protein phosphatase 2A (I2PP2A), in vitro and in situ.¹ C6 ceramide is a cell-permeable analog of naturally occurring ceramides. With a longer carbon chain than C2 ceramide , it is somewhat more hydrophobic and may more closely mimic the effects of natural ceramides.² C6 ceramide mediates many diverse biological activities including apoptosis, activation of PP2A, and inhibition of the mitochondrial respiratory chain.^2,3,4,5 It also enhances the expression of COX-2 in rat granulosa cells and stimulates the growth of bovine aortic smooth muscle cells.^6,7 C6 ceramide acts in neuronal axons to inhibit neurite growth.⁸