N-(2-Acetamido)iminodiacetic acid

한글명:N-(2-Acetamido)iminodiacetic acid

카스 번호26239-55-4

상품명:N-(2-Acetamido)iminodiacetic acid

CBNumberCB3183520

분자식C6H10N2O5

포뮬러 무게190.15

MOL 파일26239-55-4.mol

화학적 성질

녹는점	219 °C (dec.)(lit.)
끓는 점	325.64°C (rough estimate)
밀도	1.4957 (rough estimate)
증기압	0Pa at 25℃
굴절률	1.5010 (estimate)
저장 조건	room temp
용해도	1 M NaOH: 20°C에서 0.05°M, 투명, 무색
물리적 상태	가루
색상	흰색에서 거의 흰색
pH 범위	6.0 - 7.2
수소이온지수(pH)	1.5-3.0 (25℃, 0.01M in H2O)
산도 계수 (pKa)	6.6(at 25℃)
수용성	녹는
최대 파장(λmax)	λ: 260 nm Amax: 0.20 λ: 280 nm Amax: 0.05
Merck	14,147
BRN	1787181
InChIKey	QZTKDVCDBIDYMD-UHFFFAOYSA-N
LogP	-3.12 at 20℃
CAS 데이터베이스	26239-55-4(CAS DataBase Reference)
EPA	Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)- (26239-55-4)

위험 및 안전 성명

안전지침서

24/25

WGK 독일

TSCA

Yes

위험 등급

IRRITANT

HS 번호

29241900

NFPA 704:

	0
0		0

그림문자(GHS)

N-(2-Acetamido)iminodiacetic acid MSDS

N-(2-Acetamido)iminodiacetic acid 화학적 특성, 용도, 생산

개요
N-(2-Acetamido)iminodiacetic acid is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960′s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous solution is 6.0 - 7.2.
화학적 성질
N-(2-Acetamido)iminodiacetic acid is white to almost white powder
용도
N-(2-Acetamido)iminodiacetic acid (ADA) could prevents oxidation and irreversible denaturation of proteins while conducting gel electrophoresis. Furthermore studies suggest that ADA interferes with BCA and Lowry protein assays. Also ADA often chelates metal ions such as Mn(II), Cu(II), Ni(II), Zn(II), and Co(II) in a 2:1 at or below physiological pH values. Moreover, ADA is soluble in sodium hydroxide and absorbs UV radiation in the range below 260nm.
용도
N-(2-Acetamido)iminodiacetic acid is buffer for biological systems and chelator for metals.
주요 응용
ADA, or N-(2-Acetamido)iminodiacetic acid, can be used to study biological buffers and zwitterionic compounds. ADA has been used in a study to describe the application of scanning capacitively coupled contactless conductivity detection (SC(4)D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilised onto the surface of a monolithic capillary column.
Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4). Used to prepare immobilized pH gradients.
Purification Methods
Dissolve ADA in water, add one equivalent of NaOH solution (to final pH of 8-9), then acidify with HCl to precipitate the free acid. This is filtered off, washed with water and dried in vacuo. [Beilstein 4 IV 2441.]

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