서브틸리신

한글명:서브틸리신

카스 번호9014-01-1

상품명:EC 3.4.21.14

CBNumberCB1670037

분자식Unspecified

포뮬러 무게0

MOL 파일Mol file

동의어(한글)

서브틸리신

바실러스 서브틸리스 염기성 단백분해효소

화학적 성질

밀도	1.3
증기압	0Pa at 25℃
저장 조건	2-8°C
물리적 상태	가루
색상	하얀색
수용성	100g/L at 25℃
안정성	냉각
LogP	-3.1 at 25℃
CAS 데이터베이스	9014-01-1
EPA	Subtilisin (9014-01-1)

위험 및 안전 성명

위험품 표기	Xn,N
위험 카페고리 넘버	37/38-41-42-36/37/38-50-22
안전지침서	23-24-26-36/37/39-22-36/37-61-45-39
유엔번호(UN No.)	3082
OEL	STEL: 0.00006 mg/m3 [60-minute]
WGK 독일	2
RTECS 번호	UK9540000
F 고인화성물질	3-10
HS 번호	35079090
유해 물질 데이터	9014-01-1(Hazardous Substances Data)
독성	LD50 orl-rat: 3700 mg/kg FCTXAV 7,581,69

그림문자(GHS)

서브틸리신 화학적 특성, 용도, 생산

화학적 성질
These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids
용도
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
일반 설명
Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.
Safety Profile
Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
잠재적 노출
These commercial proteolytic enzymes are used in laundry detergent formulations
Purification Methods
This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
비 호환성
Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

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