-
순도시험
(1) 비소 : 이 품목을 비소시험법에 따라 시험할 때, 그 양은 4.0ppm 이하이어야 한다.
(2) 납 : 이 품목 5.0g을 취하여 원자흡광광도법 또는 유도결합플라즈마발광광도법에 따라 시험할 때, 그 양은 10.0ppm 이하이어야 한다.
(3) 기타당류 : 이 품목을 정량법에 따라 액체크로마토그래피를 행하여 D-리보오스의 유지시간의 2배까지 나타나는 피크를 관찰할 때, 시험용액 중 D-리보오스 이외의 피크면적은 전 피크 면적합계의 10.0% 이하이어야 한다.
-
확인시험
(1) 이 품목의 수용액(1→20) 2～3방울을 따뜻한 펠링시액 5mL에 가할 때, 적색의 침전이 생긴다.
(2) 이 품목의 수용액(1→25)은 좌선성이다.
-
정량법
이 품목 1.0g 및 표준품 D-리보오스 1.0g을 각각 정밀히 달아 물을 가하여 50mL씩으로 한 액을 시험용액 및 표준용액으로 한다. 시험용액 및 표준용액 각각 10μL씩을 다음의 조작조건으로 액체크로마토그래피에 주입하고 다음 계산식에 따라 D-리보오스의 함량을 구한다.
함 량(%) =무수물로 환산한 표준품의 채취량(g)/무수물로 환산한 검체의 채취량(g)×AT/AS× 100
AT : 시험용액의 피크면적
AS : 표준용액의 피크면적
조작조건
검 출 기 : 시차굴절계(RI detector)
칼 럼 : Shodex SUGAR SC1011(8×300mm) 또는 이와 동등한 것
칼럼온도 : 80℃
이 동 상 : 물
유 속 : 1.0mL/min
-
정의
이 품목은 포도당을 원료로 하여 Bacillus pumilus에 의하여 발효를 행하여 생산된 발효생산물을 분리, 정제하여 얻어진 물질로서 그 성분은 D-리보오스이다.
-
강열잔류물
이 품목 1g을 취하여 강열잔류물시험법에 따라 시험할 때, 그 양은 1.0% 이하이어야 한다.
-
개요
D-ribose is an important five-carbon monosaccharide with the chemical formula C5H10O5. It is an important constituent of ribonucleic acid (RNA) and ATP, and plays an important role in the formation of life. It is also an important pharmaceutical intermediate for the production of various nucleic acid drugs.
-
화학적 성질
D-Ribose is a five-carbon sugar with strong water solubility and sweet taste, also known as D-ribofuranose. It is used by all living cells and is an essential component in living organisms for energy production. It is widely present in the furan form.
-
출처
Reported found in boiled crab, hen egg, catfish, whitefish, haddock, stored beef, stored veal, milk, applesauce, potato, rapeseed, roasted coffee, fresh coffee and shrimp.
-
제조 방법
D-Ribose is obtained by using D-glucose as a raw material, inserting bacteria or Bacillus subtilis for fermentation, and separating and refining the fermentation product.(By fermentation technology)
-
정의
ChEBI: D-ribose is a ribofuranose having D-configuration. It is a naturally occurring monosaccharide found in the cells and particularly in the mitochondria is essential in energy production.
-
Biological Functions
D-ribose is a penta-sugar precursor of some amino acids like glutamate, histidine, proline, and arginine. This substance is transformed into piruvate, enters Krebs's cycle, and has a crucial role in energetic metabolism; it is also involved in glycogen synthesis. The non-oxidative transketolase PPP reaction can be directly synthesized by transferring a keto group to glyceraldehyde 3-phoshate from fructose 6-phosphate. Both these substrates are intermediate products of glycolysis[1].
-
생물학적 활성
D-ribose is a natural sugar that our bodies produce for a variety of purposes most notably encrgy in the form of ATP(adenosine triphosphate), which powers every cell in our body. Ribose is crucial for the synthesis of energy (ATP) and without it, your cells run out of energy and the health of the cell is compromised. Supplementing with ribose improves cell function by restoring energy.
One of the most vital organs to suffer energy depletion is the heart. That is why so much of the research on D-ribose has focused on the impact of supplement ribose in heart disease. Although ribose is classified as a sugar, when taken orally, it does not have any impact on blood sugar like glucose. Instead, it is used for energy production and energy recovery. Every cell in the body has the capacity to make ribose. But more ribose may be needed than the cell can produce when cells are metabolically stressed, such as with strenuous exercise or disease. When the cells are metabolically stressed they tend to consume more glucose rather than producing more ribose. This can dramatically affect recovery.
-
부작용
D-ribose is a dietary supplement approved by the US FDA. It is generally considered safe for dosage of ribose is less than 15g/d. However, possible side effects include diarrhea, stomach discomfort, nausea, headache, and low blood sugar.
-
Purification Methods
Crystallise -D(-)-ribose from aqueous 80% EtOH, dry it under vacuum at 60o over P2O5 and store it in a vacuum desiccator. It exhibits a complex mutarotation with : [] D 10 -23.1o (1.5minutes), -21.3o (5minutes), -19.5o (10minutes), -19.1o (30minutes), -21.2o (60minutes), -23.1o (120minutes), -23.7o (300minutes), (c 4.5, H2O) [Phelps et al. J Am Chem Soc 56 748 1934]. 1H NMR in D2O at 44o shows 17% -pyranose, 59% -pyranose, 9% -furanose and 15% -furanose forms with furanose -H at 5.34ppm (J 3.0Hz) and -H at 5.31 (J 1.7Hz) [Angyal Adv Carbohydr Chem 42 15 1984, Angyal & Pickles Aust J Chem 25 1711 1972]. The phenylhydrazone crystallises from aqueous pyridine in yellow needles, m 163-164o, and the benzylphenylhydrazone has m 127-128o [Snowden J Am Chem Soc 72 808 1950.] [Beilstein 1 IV 4211.]
