ニトロブルーテトラゾリウム

危険有害性情報のコード(GHS)

• 絵表示(GHS)

  

• 注意喚起語

  Danger

• 危険有害性情報

  H315：皮膚刺激

  H319：強い眼刺激

  H332：吸入すると有害

• 注意書き

  P201：使用前に取扱説明書を入手すること。

  P261：粉じん/煙/ガス/ミスト/蒸気/スプレーの吸入を避ける こと。

  P264：取扱い後は皮膚をよく洗うこと。

  P264：取扱い後は手や顔をよく洗うこと。

  P280：保護手袋/保護衣/保護眼鏡/保護面を着用するこ と。

  P308+P313：暴露または暴露の懸念がある場合：医師の診断/手当てを 受けること。

ニトロブルーテトラゾリウム 価格

MSDS Information

• Nitrotetrazolium blue chloride

ニトロブルーテトラゾリウム 化学特性,用途語,生産方法

• 外観

  黄色結晶性粉末

• 溶解性

  水に溶け、アセトンにほとんど溶けない。50mg/10ml(熱水), 100mg/10ml(熱メチルアルコール), 50mg/50ml(トリスバッファー, pH 7.4)エタノールにはメタノールほど溶けない。アセトン, エーテルなど有機溶媒には全く溶けない。純度が高くなるほど冷水、冷エタノールに溶けにくくなる。加温すれば可溶。

• 用途

  臨床検査薬。こはく酸脱水素酵素、クレアチンホスホキナーゼの検出ならびに定量。

• 効能

  診断補助薬

• 説明

  Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

• 化学的特性

  NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.

• 使用

  Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

• 主な応用

  Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
  It has been used as a substrate for western blot detection.
  It has been used for the staining of histological tissue sections.
  Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
  It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
  Further, it is used in immunology for detection of alkaline phosphatase.

• 定義

  ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).

• 一般的な説明

  Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

• 危険性

  Moderately toxic by ingestion.

• Biochem/physiol Actions

  Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

• 安全性プロファイル

  Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.

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