ChemicalBook > CAS DataBase List > EC 2.7.1.40

EC 2.7.1.40

CAS No.9001-59-6
Chemical Name:EC 2.7.1.40
CBNumber:CB9396847
Molecular Formula:NULL
Formula Weight:0
MOL File:Mol file

EC 2.7.1.40 Property

storage temp. -20°C
form powder
color white
PH ~6
biological source rabbit muscle
Water Solubility Soluble in water ( 1mg/ml).
Specific Activity 350-600units/mg protein
EPA Substance Registry System Kinase (phosphorylating), pyruvate (9001-59-6)
UNSPSC Code 12352204
NACRES NA.54

Safety

WGK Germany :3
F :10-21
TSCA :Yes
HS Code :35079090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Hazard statements
Precautionary statements

EC 2.7.1.40 Price More Price(17)

Brand: Sigma-Aldrich(India)
Product number: P9136
Product name : Pyruvate Kinase from rabbit muscle
Purity: Type III, lyophilized powder, 350-600?units/mg protein
Packaging: 1000UNITS
Price: ₹6005.1
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: P9136
Product name : Pyruvate Kinase from rabbit muscle
Purity: Type III, lyophilized powder, 350-600?units/mg protein
Packaging: 5000UNITS
Price: ₹21234.3
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: SRP0414
Product name : PKLR Var2 active human
Purity: recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Packaging: 20μG
Price: ₹57498
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: P9136
Product name : Pyruvate Kinase from rabbit muscle
Purity: Type III, lyophilized powder, 350-600?units/mg protein
Packaging: 25000UNITS
Price: ₹55255.8
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: SRP0413
Product name : PKLR Var1 active human
Purity: recombinant, expressed in E. coli, ≥43% (SDS-PAGE)
Packaging: 20μG
Price: ₹57498
Updated: 2022/06/14
Buy: Buy

EC 2.7.1.40 Chemical Properties,Usage,Production

Chemical Properties White powder
Uses Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.
Uses Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.
Uses Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.
General Description Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M₁, and M₂. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M₁ are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M₂ is localized in the nucleus of the cells.
Biochem/physiol Actions Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ～7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Purification Methods These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]