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HEPPS

CAS No.16052-06-5
Chemical Name:HEPPS
CBNumber:CB9310937
Molecular Formula:C9H20N2O4S
Formula Weight:252.33
MOL File:16052-06-5.mol

HEPPS Property

Melting point 237-239 °C(lit.)
Density 1.2684 (rough estimate)
refractive index 1.6800 (estimate)
storage temp. Sealed in dry,Room Temperature
solubility H₂O: 1 M at 20 °C, clear, colorless
form Powder/Solid
color White
PH 5.0-6.5 (25℃, 0.1M in H2O)
pka 8.0(at 25℃)
PH Range 7.3 - 8.7
Water Solubility Soluble in water. 25.2 g/L (20°C)
λmax λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.045
BRN 3957385
CAS DataBase Reference 16052-06-5(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII 8JN5PQW99C
EPA Substance Registry System 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5)
Absorption ≤0.1 at 260 in H2O at 1M
≤0.1 at 280 in H2O at 1M
UNSPSC Code 12161700
NACRES NA.25

Safety

Hazard Codes :Xi
Risk Statements :36/37/38-22
Safety Statements :24/25-36-26
WGK Germany :3
RTECS :TM2649130
TSCA :Yes
HazardClass :IRRITANT
HS Code :29335995
Toxicity :quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982.

NFPA 704:

	1
2		1

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H302-H315-H319-H332-H335
Precautionary statements P280-P305+P351+P338-P310

HEPPS Price More Price(19)

Brand: Sigma-Aldrich(India)
Product number: E9502
Product name : EPPS
Purity: ≥99.5% (titration)
Packaging: 10G
Price: ₹2554.7
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: E9502
Product name : EPPS
Purity: ≥99.5% (titration)
Packaging: 25G
Price: ₹4156.8
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: E9502
Product name : EPPS
Purity: ≥99.5% (titration)
Packaging: 100G
Price: ₹8941.45
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: E9502
Product name : EPPS
Purity: ≥99.5% (titration)
Packaging: 250G
Price: ₹16096.78
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: E9502
Product name : EPPS
Purity: ≥99.5% (titration)
Packaging: 1KG
Price: ₹47575.88
Updated: 2022/06/14
Buy: Buy

HEPPS Chemical Properties,Usage,Production

Chemical Properties white crystalline powder
Uses HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.
Definition ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine.
General Description HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.
Flammability and Explosibility Not classified