Description
3''-Dephosphocoenzyme A is an intermediate in the biosynthesis of coenzyme A (CoA; Item Nos.
16147 |
21499 |
21722) from pantothenic acid (vitamin B
5; ). It is phosphorylated by CoA synthase in humans to form CoA. 3''-Dephosphocoenzyme A can be used as a transcription initiator in the synthesis of CoA-RNA by
in vitro transcription.
Uses
Dephosphocoenzyme A is the direct precursor of coenzyme A. It is used for the synthesis of coenzyme A by bifunctional CoA Synthase (CoASy, 4′-phosphopantetheine adenylyltransferase/dephospho-CoA kinase) which performs the transfer of the γ-phosphate of ATP to dephosphocoenzyme A.
Definition
ChEBI: An adenosine 5'-phosphate that is coenzyme A in which the phosphate group at position 3' has been replaced by a hydrogen atom. It is an intermediate metabolite in pantothenate and CoA biosynthesis
Biochem/physiol Actions
3′-Dephosphocoenzyme A (DepCoA), also called desphosphoCoA (dpCoA), is synthesized from 4-phosphopantetheine by the enzyme phosphopanthetheine adenylyltransferase. It is an intermediate in coenzyme A biosynthetic pathway. In the presence of dephospho-CoA kinase (CoaE), dephosphocoenzyme A is converted to coenzyme A. 3′-Dephosphocoenzyme shares adenosine-diphosphate substructure as like the purines and is a non-canonical nucleotide and is an initiator of transcription initiation. It is routinely used in in vitro transcription studies to initiate RNA synthesis.
IC 50
Human Endogenous Metabolite
References
[1] HUANG F. Efficient incorporation of CoA, NAD and FAD into RNA by in vitro transcription.[J]. Nucleic Acids Research, 2003, 31 3: e8. DOI:
10.1093/nar/gng008[2] KRISHNA SAPKOTA Faqing H. Efficient one-pot enzymatic synthesis of dephospho coenzyme A[J]. Bioorganic Chemistry, 2018, 76: Pages 23-27. DOI:
10.1016/j.bioorg.2017.10.012
