ChemicalBook > CAS DataBase List > 2′,7′-Dichlorodihydrofluorescein Diacetate
2′,7′-Dichlorodihydrofluorescein Diacetate
2′,7′-Dichlorodihydrofluorescein Diacetate
- CAS No.4091-99-0
- Chemical Name:2′,7′-Dichlorodihydrofluorescein Diacetate
- CBNumber:CB8497619
- Molecular Formula:C24H16Cl2O7
- Formula Weight:487.29
- MOL File:4091-99-0.mol
2′,7′-Dichlorodihydrofluorescein Diacetate Property
- Melting point 209-210 °C(lit.)
- Boiling point 593.5±50.0 °C(Predicted)
- Density 1.463±0.06 g/cm3(Predicted)
- storage temp. -20°C
- solubility DMF: soluble
- pka 3.79±0.36(Predicted)
- form Crystalline solid
- color White to off-white
- BRN 4610273
- Stability Light Sensitive
- EPA Substance Registry System 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)
- UNSPSC Code 12352202
- NACRES NA.77
Safety
- Hazard Codes :Xi
- Risk Statements :36/37/38
- Safety Statements :22-24/25-26
- WGK Germany :3
- F :10-21
- HS Code :29329990
-
NFPA 704:
0 2 0
-
Symbol(GHS)
- Signal wordWarning
- Hazard statements H302-H315-H319
- Precautionary statements P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
2′,7′-Dichlorodihydrofluorescein Diacetate Price
More Price(7)
- Brand: Sigma-Aldrich(India)
- Product number: D6883
- Product name : 2′,7′-Dichlorofluorescin diacetate
- Purity: ≥97%
- Packaging: 50MG
- Price: ₹4784.65
- Updated: 2022/06/14
- Buy: Buy
- Brand: Sigma-Aldrich(India)
- Product number: D6883
- Product name : 2′,7′-Dichlorofluorescin diacetate
- Purity: ≥97%
- Packaging: 250MG
- Price: ₹18651.48
- Updated: 2022/06/14
- Buy: Buy
- Brand: Sigma-Aldrich(India)
- Product number: 287810
- Product name : 2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem
- Purity: Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of
- Packaging: 100MG
- Price: ₹13470.01
- Updated: 2022/06/14
- Buy: Buy
- Brand: ottokemi
- Product number: D 9474
- Product name : 2′,7′-Dichlorofluorescin diacetate, ≥97%
- Purity:
- Packaging: 50mg
- Price: ₹8649
- Updated: 2022/05/26
- Buy: Buy
- Brand: ottokemi
- Product number: D 9474
- Product name : 2′,7′-Dichlorofluorescin diacetate, ≥97%
- Purity:
- Packaging: 250mg
- Price: ₹39069
- Updated: 2022/05/26
- Buy: Buy
2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties,Usage,Production
-
Description
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-
catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1). - Chemical Properties white to yellow-beige or pale pink powder
-
Uses
2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. - Uses 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.
- Uses Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.
- General Description Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.
- Biochem/physiol Actions Cell permeable: yes
- storage Store at -20°C
2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials
Raw materials
Preparation Products
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4091-99-0, 2′,7′-Dichlorodihydrofluorescein DiacetateRelated Search:
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- 4091-99-0
- H2-DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] - 100 mg
- H2-DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] - 100 mg
- H2DCFDA, 10 mM in DMSO
- 2'', 7''-Dichlorofluorescin diacetate
- DCFH-DA reactive oxygen species ROS fluorescent probe
- 2',7'-Dichlorofluorescein diacetate
- 2',7'-dichlorofluorescein oxalate
- 2',7'-Dichlorodihydrofluorescein diacetate|||DCFH-DA
- 2',7'-Dichlorodihydrofluorescein diacetate
- 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoicaci
- DCFH
- DCFH-DA
- Dichlorofluorescin diacetate
- 2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] *CAS 4091-99-0*
- 2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem
- 187263
- 2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97%
- 2′,7′-Dichlorofluorescin diacetate, ≥97%
- H2DCFDA / DCFH-DA
- DCFH | DCFH-DA
- H2DCFDA[2',7'-DICHLORODIHYDROFLUORESCEINDIACETATChemicalbookE]
- Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
- 2-[3,6-bis(acetyloxy)-2,7-dichloro-9h-xanthen-9-yl]-benzoicaci
- 2' 7'-DICHLOROFLUORESCIN DIACETATE FOR&