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Nitrotetrazolium blue chloride

CAS No.298-83-9
Chemical Name:Nitrotetrazolium blue chloride
CBNumber:CB8465489
Molecular Formula:C40H30ClN10O6+
Formula Weight:782.19
MOL File:298-83-9.mol

Nitrotetrazolium blue chloride Property

Melting point 200°C
Density 1.5521 (rough estimate)
refractive index 1.7350 (estimate)
storage temp. 2-8°C
solubility methanol: 50 mg/mL, clear, very strongly yellow
form Powder/Solid
color Yellow
PH 4.5-5.5
Water Solubility Soluble in water, ethanol and methanol.
Sensitive Light Sensitive
λmax 256 nm
BRN 4115923
Stability Stable, but store cold.
Biological Applications Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
FDA UNII X44P41F7ZK
EPA Substance Registry System 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)
UNSPSC Code 41116130
NACRES NA.77

Safety

Hazard Codes :Xn,Xi
Risk Statements :22-68/20/21/22-20/21/22-36-36/37/38-41
Safety Statements :36-45-36/37-26-39
WGK Germany :3
RTECS :XF8045000
F :8-10-23
TSCA :Yes
HS Code :32041900
Toxicity :LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

NFPA 704:

	1
2		0

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordDanger
Hazard statements H315-H319-H332-H360D
Precautionary statements P201-P261-P264-P280-P304+P340+P312-P308+P313

Nitrotetrazolium blue chloride Price More Price(29)

Brand: Sigma-Aldrich(India)
Product number: N6876
Product name : Nitrotetrazolium Blue chloride
Purity: ≥90.0% (HPLC)
Packaging: 50MG
Price: ₹4481.55
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: N6876
Product name : Nitrotetrazolium Blue chloride
Purity: ≥90.0% (HPLC)
Packaging: 100MG
Price: ₹6495
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: N6876
Product name : Nitrotetrazolium Blue chloride
Purity: ≥90.0% (HPLC)
Packaging: 250MG
Price: ₹10435.3
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: N6876
Product name : Nitrotetrazolium Blue chloride
Purity: ≥90.0% (HPLC)
Packaging: 10X10MG
Price: ₹13747.75
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: N6876
Product name : Nitrotetrazolium Blue chloride
Purity: ≥90.0% (HPLC)
Packaging: 500MG
Price: ₹20166.98
Updated: 2022/06/14
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Nitrotetrazolium blue chloride Chemical Properties,Usage,Production

Description Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.
Chemical Properties NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.
Uses Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.
Application Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.
Definition ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).
General Description Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.
Hazard Moderately toxic by ingestion.
Biochem/physiol Actions Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.
Safety Profile Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.
storage +4°C