Uses
Nitrate Reductase, Arabidopsis thaliana is the isomer of NAD(P)H-Nitrate reductase (HY-P2996). NAD(P)H-Nitrate reductase is isolated from Aspergillus niger that catalyses the reduction of nitrate to nitrite via a two-electron transfer. In plants，the electron donor for Nitrate reductase is NADPH is NADH:Nitrate reductase and a bispecific NAD(P)H: Nitrate reductase[1].
General Description
Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.
Biochem/physiol Actions
Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.
References
[1] Campbell WH. Structure and function of eukaryotic NAD(P)H:nitrate reductase. Cell Mol Life Sci. 2001 Feb;58(2):194-204. DOI:
10.1007/PL00000847
