Uses
A semi-flexible linker useful for PROTAC development for targeted protein degradation. Incorporation of rigidity into the linker region of PROTACs may impact degradation kinetics as well as drug design (absorption; distribution; metabolism; excretion; and toxicity; ADMET) properties of PROTACs.
Technology Spotlight:
Degrader Building Blocks for Targeted Protein Degradation
Protein Degrader Building Blocks
Synthesis
General procedure for the synthesis of 2-(N-Boc-4-piperidinyl)ethylamine from 1-Boc-4-(cyanomethyl)piperidine: tert-butyl 4-(cyanomethyl)piperidine-1-carboxylate (20 g, 89.29 mmol) and NH4OH (9 mL) were dissolved in methanol (200 mL) and Raney Ni (6 g, 101.69 mmol) was added. The reaction was carried out at 50°C for 5 h under H2 pressure of 50 psi. Upon completion of the reaction, the mixture was filtered through a diatomaceous earth pad and the filtrate was concentrated to afford the target product 2-(N-Boc-4-piperidinyl)ethylamine (20.5 g, 100%) as a colorless oil.1H NMR (400 MHz, CDCl3) δ 1.06-1.19 (m, 2H), 1.44 (d, J = 7.41 Hz, 2H), 1.47 (s 9H), 1.50-1.58 (m, 1H), 1.66 (d, J = 12.49 Hz, 2H), 2.09 (br.s., 2H), 2.70 (t, J = 12.29 Hz, 2H), 2.75-2.83 (m, 2H), 4.08 (br.s., 2H).
References
[1] Bioorganic and Medicinal Chemistry Letters, 2017, vol. 27, # 21, p. 4805 - 4811
