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7-AMINOACTINOMYCIN D

CAS No.7240-37-1
Chemical Name:7-AMINOACTINOMYCIN D
CBNumber:CB7145520
Molecular Formula:C62H87N13O16
Formula Weight:1270.43
MOL File:7240-37-1.mol

7-AMINOACTINOMYCIN D Property

Melting point 252～253℃
Boiling point 1400.8±65.0 °C(Predicted)
Density 1.44
Flash point 801℃
storage temp. -20°C
solubility chloroform: 1 mg/mL, clear, red
pka 8.90±0.70(Predicted)
form powder
color red to dark purple
BRN 5915844
Stability Stable for 1 year from date of purchase as supplied. Solutions should be made fresh daily.
FDA UNII DXS32PH95R
UNSPSC Code 12352200
NACRES NA.77

Safety

Hazard Codes :T+,T
Risk Statements :26/27/28-36/37/38
Safety Statements :26-28-36/37-45
RIDADR :UN 3462 6.1/PG 2
WGK Germany :3
RTECS :AU1579000
F :8-10-21
HazardClass :6.1(a)
PackingGroup :II
HS Code :29419090

NFPA 704:

	0
4		0

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordDanger
Hazard statements H300
Precautionary statements P264-P270-P301+P310-P405-P501

7-AMINOACTINOMYCIN D Price More Price(3)

Brand: Sigma-Aldrich(India)
Product number: A9400
Product name : 7-Aminoactinomycin D
Purity: ~97% (HPLC), powder
Packaging: 1MG
Price: ₹21953.1
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: SML1633
Product name : 7-AAD Ready Made Solution
Purity: 1?mg/mL
Packaging: 1ML
Price: ₹29007.3
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: A9400
Product name : 7-Aminoactinomycin D
Purity: ~97% (HPLC), powder
Packaging: 5MG
Price: ₹86535.05
Updated: 2022/06/14
Buy: Buy

7-AMINOACTINOMYCIN D Chemical Properties,Usage,Production

Description 7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells. It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC. 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.
Chemical Properties Purple powder
Uses 7-Aminoactinomycin D is a fluorescent DNA dye for apoptosis and flow cytomtery studies.
Uses Fluorescent DNA stain. A featured product for Apoptosis research.
Definition ChEBI: 7-aminoactinomycin D is a chromopeptide. It has a role as a fluorochrome.
General Description Free form:
Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm
Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm
DNA Complex:
Excitation: 543 nm; 555 nm
Emission: 655 nm; 665 nm
Biochem/physiol Actions Primary Targetleukemias and sarcomas
storage Store at -20°C
References 1) Telieps?et al.?(2013)?Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection, Eur. J. Immunol. 43 1499 2) Schmid?et al.?(2007)?Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D, Nat. Protoc.?2?187 3) Toba?et al.?(2000) Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY, Eur.J.Haematol. 64 10 4) Lecoeur?et al.?(1997)?Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods, J. Immunol. Methods?209?111 5) Su?et al.?(1996)?Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect, J. Immunol.?156?4198