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Sodium deoxycholate

CAS No.302-95-4
Chemical Name:Sodium deoxycholate
CBNumber:CB4452346
Molecular Formula:C24H41NaO4
Formula Weight:416.58
MOL File:302-95-4.mol

Sodium deoxycholate Property

Melting point 357-365 °C
alpha 44 º (c=2, H2O)
Density 1.012 at 20.07℃
bulk density 200kg/m3
vapor pressure 0Pa at 20℃
Flash point >110°C (own results)
storage temp. room temp
solubility water: soluble1 gm in 10 ml, clear, colorless to very faintly yellow
form Powder
pka 6.2
color Yellow-tan
PH 7.5-9.0 (20g/l, H2O, 20℃)
Odor Odorless
PH Range 7 - 10
optical activity [α]20/D +44±2°, c = 2% in H2O
biological source ox bile
Water Solubility 330 g/L (15 ºC)
BRN 3581950
LogP 5.35 at 22℃ and pH5.7
Surface tension 41.8mN/m at 1g/L and 20℃
CAS DataBase Reference 302-95-4(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII 436LS6U35Y
EPA Substance Registry System Cholan-24-oic acid, 3,12-dihydroxy-, monosodium salt, (3.alpha.,5.beta.,12.alpha.)- (302-95-4)
UNSPSC Code 41106212
NACRES NA.74

Safety

Hazard Codes :Xn
Risk Statements :22-37
Safety Statements :22-24/25
WGK Germany :3
RTECS :FZ2250000
F :3-10
TSCA :Yes
HS Code :29181930
Toxicity :LD50 orally in Rabbit: 1370 mg/kg

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H302
Precautionary statements P264-P270-P301+P312-P501

Sodium deoxycholate Price More Price(21)

Brand: Sigma-Aldrich(India)
Product number: D6750
Product name : Sodium deoxycholate
Purity: ≥97% (titration)
Packaging: 10G
Price: ₹3210
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: SRE0046
Product name : Sodium deoxycholate
Purity: Suitable for manufacturing of diagnostic kits and reagents
Packaging: 100G
Price: ₹24551.1
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: D6750
Product name : Sodium deoxycholate
Purity: ≥97% (titration)
Packaging: 25G
Price: ₹5610
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: SRE0046
Product name : Sodium deoxycholate
Purity: Suitable for manufacturing of diagnostic kits and reagents
Packaging: 500G
Price: ₹49156.33
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: D6750
Product name : Sodium deoxycholate
Purity: ≥97% (titration)
Packaging: 100G
Price: ₹15890
Updated: 2022/06/14
Buy: Buy

Sodium deoxycholate Chemical Properties,Usage,Production

Chemical Properties Sodium deoxycholate is white to cream crystalline powder
Uses Sodium deoxycholate is an ionic detergent for the solubilization of membrane-bound proteins.
Uses Sodium deoxycholate is used as a choleretic and an anionic detergent. It is useful for the extraction of membrane receptors, plasma membrane proteins, nuclei isolation and solubilizes fats as well as membrane components. It acts as an intermediate for the production of corticosteroids and microbiological diagnostic media. In addition, it acts as a catalyst in the preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.
Uses Sodium deoxycholate is used as a component in cell lysis buffers.
Definition ChEBI: Sodium deoxycholate is a bile acid salt. It contains a deoxycholate.
General Description Sodium deoxycholate is a bile salt and an ionic detergent. Bile salts work along with lipids/fats/cholesterol and form mixed micelles in the intestine. These micelles help in fat digestion and absorption through the intestinal wall.
Biological Activity Sodium deoxycholate (SDC) is an anionic detergent which has been used both to destroy infectious viruses and to fractionate viral components. Sodium deoxycholate begins to bind to the virus at less than 0.1 mM free equilibrium concentration. It causes lysis of the viral membrane at 0.9 +/- 0.1 mM free equilibrium concentration when 2.2 +/- 0.2 - 103 mol of sodium deoxycholate are bound per mol of the virus. The liberation of proteins from the membrane begins at 1.5 +/- 0.1 mM sodium deoxycholate, and the proteins released are virtually free from phospholipids above 2.0 mM sodium deoxycholate. The overall mechanism of sodium deoxycholate solubilization of the viral membrane resembles that of Triton X-100 and sodium dodecyl sulphate, except that with sodium deoxycholate, the various stages of membrane disruption occur at about 10-fold higher equilibrium free detergent concentrations. At sodium deoxycholate concentrations higher than 2.3 mM, the viral spike glycoproteins can be separated by sucrose gradient centrifugation or gel filtration into constituent polypeptides E1, E2 and E3[1-2].
Biochem/physiol Actions Solubilizes fats for absorption in the intestines.
Safety Profile Poison by intraperitoneal and intravenous routes. Moderately toxic by ingestion and subcutaneous routes. Experimental reproductive effects. Mutation data reported. When heated to decomposition it emits toxic fumes of NasO.
Purification Methods Recrystallise it from EtOH and dry it in an oven at 100o. The solution is freed from soluble components by repeated extraction with acid-washed charcoal. [Beilstein 10 IV 1608.]
References [1] A Helenius. “Solubilization of the Semliki Forest virus membrane with sodium deoxycholate.” Biochimica et biophysica acta 436 2 (1976): 319–34.
[2] A E Auletta, M L Marlowe. “Effect of sodium deoxycholate on rubella virus.” Applied microbiology 16 10 (1968): 1624.