MMP-1/MMP-9 substrate, fluorogenic

Description

Dnp-P-Cha-G-Cys(Me)-HA-K(Nma)-NH₂ is a fluorogenic substrate for matrix metalloproteinase-1 (MMP-1) and MMP-9. Upon cleavage by MMP-1 or MMP-9, N-methylanthranilic acid (Nma) is unquenched and its fluorescence can be used to quantify MMP activity. Nma displays excitation/emission spectra of 340/440 nm, respectively.

Uses

MMP-1/MMP-9 Substrate, Fluorogenic is a fluorogenic substrate for MMP-1 and MMP-9[1].Ex/Em = 340/440 nm

References

[1] B T Frank, et al. Mast Cell Tissue Inhibitor of Metalloproteinase-1 Is Cleaved and Inactivated Extracellularly by α-Chymase. J Immunol.2001 Feb 15;166(4):2783-92. DOI:10.4049/jimmunol.166.4.2783

Sequene

Dnp-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Nma)-NH2