(S)-Bromoenol lactone-d7

Description

(S)-Bromoenol lactone-d7 ((S)-BEL-d7) is intended for use as an internal standard for the quantification of (S)-BEL by GC- or LC-MS. The phospholipases are an extensive family of lipid hydrolases that function in cell signaling, digestion, membrane remodeling, and as venom components.1 The calcium-independent phospholipase A2 (iPLA2) are a PLA2 subfamily closely associated with the release of arachidonic acid in response to physiologic stimuli. (S)-Bromoenol lactone ((S)-BEL) is an irreversible, chiral, mechanism-based inhibitor of iPLA2β that inhibits the vasopressin-induced release of arachidonate from cultured rat aortic smooth muscle (A10) cells with an IC50 value of 2 µM.2 (S)-BEL is more than 1,000-fold selective for iPLA2 versus cPLA2, and is 10-fold selective for iPLA2β versus iPLA2γ.WARNING This product is not for human or veterinary use.

References

[1] SCHEUER W. Phospholipase A2–regulation and inhibition.[J]. Klinische Wochenschrift, 1989, 67 3: 153-159. DOI: 10.1007/bf01711343
[2] C. M. JENKINS. Identification of Calcium-independent Phospholipase A2 (iPLA2) β, and Not iPLA2γ, as the Mediator of Arginine Vasopressin-induced Arachidonic Acid Release in A-10 Smooth Muscle Cells[J]. The Journal of Biological Chemistry, 2002, 26 1: 32807-32814. DOI: 10.1074/jbc.m202568200