Description
BAPTA free acid (85233-19-8) is a selective extracellular Ca2+ chelator. It is a widely used calcium sponge for chelating Ca2+outside of intact cells.1 It’s a useful tool to probe the involvement of extracellular Ca2+ in cellular signaling.2,3 Non-cell-permeable.
Chemical Properties
White Solid
Uses
Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels.
Uses
As calcium chelator; 19F labelled forms for measurement of intracellular calcium.
Uses
BAPTA is a highly selective calcium chelating reagent. Lipophilic diesters of the chelating agent 1,2-Bis(2 aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) are used for inhibition of proteolytic activities of certain metalloproteinases, calpain and TACE.
Definition
ChEBI: A polyamino carboxylic acid in which bis(carboxymethyl)nitrilo groups are bonded to C-2 and C-2' of 1,1'-[ethane-1,2-diylbis(oxy)]dibenzene.
General Description
In nature 1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetra acetic acid (BAPTA) is a main metabolic product of BAPTA -AM (BAPTA-tetra(actoxymethyl ester), a neuro protective agent in cerebral ischemia, in rats.BAPTA is an intercellular Ca
2+ chelator.
Biological Activity
Selective calcium chelator.
References
[1] ALBERTO MUÑOZ. Different Stress-Induced Calcium Signatures Are Reported by Aequorin-Mediated Calcium Measurements in Living Cells of Aspergillus fumigatus.[J]. PLoS ONE, 2015: e0138008. DOI:
10.1371/journal.pone.0138008[2] K. WOZNIAK. Extracellular Ca2+ Is Required for Fertilization in the African Clawed Frog, Xenopus laevis[J]. PLoS ONE, 2017. DOI:
10.1371/journal.pone.0170405[3] MIROSLAVA DIDIASOVA. STIM1/ORAI1-mediated Ca2+ Influx Regulates Enolase-1 Exteriorization.[J]. The Journal of Biological Chemistry, 2015: 11983-11999. DOI:
10.1074/jbc.m114.598425
