3':5'-Cyclic-nucleotide 5'-nucleotidohydrolase

Uses

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Uses

For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.

General Description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca²⁺ second-messenger systems.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Clinical Use

Phosphodiesterase-3 (PDE3) is an enzyme responsible for degradation of cAMP to AMP in platelets and blood vessels. Selective cAMP PDE3 inhibitors, such as dipyridamole and cilostazol , inhibit the degradation of cAMP, thereby increasing cellular concentration of cAMP and leading to inhibition of platelet aggregation and vasodilation.