Description
Latrunculin B (76343-94-7) inhibits actin polymerization and disrupts microfilament organization.1 Significantly more potent than cytochalasins in the disruption of microfilament mediated processes2, causes shortening and thickening of stress fibers. Active in cell culture.3,4Effective doses for latrunculin B vary depending upon cell type but are frequently in the low micromolar range. Note: Latrunculin B is slowly inactivated by fetal bovine serum.
Uses
In elucidation of molecular mechanisms of motile processes.
Uses
Latrunculin B is an actin polymerization inhibitor.
Uses
Latrunculin B from
Latruncula magnifica has been used for actin depolymerisation in various experiments. It has also been used as a component in bone marrow-derived dendritic cell (BMDC) complete medium, to study the formation of tubules from phagosomes in dendritic cells.
Definition
ChEBI: A macrolide consisting of a 14-membered bicyclic lactone attached to the rare 2-thiazolidinone moiety. It is obtained from the Red Sea sponge Latrunculia magnifica.
General Description
Latrunculin B is a macrocyclic toxin, which is produced by
Latruncula magnifica.
Biochem/physiol Actions
Primary TargetActin polymerization
References
[1] MARTINE COUÉ . Inhibition of actin polymerization by latrunculin A[J]. FEBS Letters, 1987, 213 2: Pages 316-318. DOI:
10.1016/0014-5793(87)81513-2[2] ILAN SPECTOR. Latrunculins—novel marine macrolides that disrupt microfilament organization and affect cell growth: I. Comparison with cytochalasin D[J]. Cytoskeleton, 1989, 13 3: 127-144. DOI:
10.1002/cm.970130302[3] Effects of cytochalasin D and latrunculin B on mechanical properties of cells
[4] BOYOUNG CHA. The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP.[J]. Journal of cell science, 2004, 117 Pt 15: 3353-3365. DOI:
10.1242/jcs.01180[5] HERRERA F. Cell cultures, transfection and treatments v1[J]. protocols.io, 2021, 21 1. DOI:
10.17504/protocols.io.77ehrje
