DISPASE

Uses

Pronase E (from Streptomyces griseus) 4,000,000 PU/g for biochemistry EC 3.4.24.4. CAS 9036-06-0, pH 6-7 (10g/l, HO, 20°C).

General Description

from Streptomyces griseus 4,000,000 PU/g for biochemistry EC 3.4.24.4

Description

Neutral Protease (Dispase^®) is isolated from Bacillus polymyxa and is a mild proteolytic enzyme that separates the epidermis from dermis by cleaving the basement membrane zone of the skin while preserving the viability of epithelial sheets. This enzyme is effective at cleaving fibronectin and type IV collagen in the lamina densa of skin. It will not cleave serum albumin, transferrin, laminin, type V collagen, or anchoring fibrils containing type VII collagen. As described in the expired U.S. Patent 4,304,866, Neutral Protease (Dispase^®) is activated by metallic ions such as Ca⁺⁺, Mn⁺⁺, Mg⁺⁺, Fe⁺⁺, Fe⁺⁺⁺, and Al⁺⁺⁺ and is inhibited by metal-chelating agents such as ethylenediaminetetraacetic acid (EDTA), citric acid, o-phenanthroline, 2,2'-dipyridyl and sodium fluoride, and oxidizing agents such as N-bromosuccinimide (NBS) and iodine. Neutral Protease (Dispase^®) is used at various concentrations for tissue dissociation, generally ranging from 0.6 to 2.4 U/ml for various time points depending on the desired effect. Concentrations higher than 2.4 U/ml are not recommended.